PhosphoDetect Anti-RhoA (pSer188) Rabbit pAb

Analysis Note

Positive ControlRhoA GST fusion protein

Application

Immunoblotting (1:1000)ELISA (1:2000)

General description

This PhosphoDetect™ Anti-RhoA (pSer¹⁸⁸) Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of RhoA (pSer¹⁸⁸).

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~22 kDa RhoA protein when expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.

Recognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.

Immunogen

a synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH

Human

Legal Information

TWEEN is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Ellerbroek, S.M., et al. 2003. J. Biol. Chem.278, 19023.Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.Dong, J.M., et al. 1998. J. Biol. Chem.273, 22554.Lang, P., et al. 1996. EMBO J.15, 510.

Does not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween^® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1 mg/ml BSA, 50% glycerol.

Reconstitution

Do not aliquot.

Warning

Toxicity: Standard Handling (A)